木犀草素抑制与前列腺癌PC3细胞共培养的内皮细胞增殖、迁移及其分子机制

平耀东, 梁泰刚, 张艳华

中国药学杂志 ›› 2018, Vol. 53 ›› Issue (7) : 508-512.

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中国药学杂志
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中国药学杂志 ›› 2018, Vol. 53 ›› Issue (7) : 508-512. DOI: 10.11669/cpj.2018.07.006
论 著

木犀草素抑制与前列腺癌PC3细胞共培养的内皮细胞增殖、迁移及其分子机制

  • 平耀东1, 梁泰刚2, 张艳华1*
作者信息 +

Inhibition Effect of Luteolin on the Proliferation and Migration of the Endothelial Cells Co-Cultured with Cancer Cells and Its Molecular Mechanism

  • PING Yao-dong1, LIANG Tai-gang2, ZHANG Yan-hua1*
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摘要

目的 探讨木犀草素对与前列腺癌PC3细胞共培养的内皮细胞增殖、迁移的影响及其分子机制。方法 原代分离培养人脐静脉内皮细胞(HUVECs)并用VE-钙黏素的表达进行鉴定。使用Transwell系统建立肿瘤-内皮细胞共培养体系。检测不同浓度[0 (共培养对照组)、20与50 μmol·L-1]木犀草素对共培养系统中HUVECs细胞增殖、迁移的影响。加设不含前列腺癌PC3细胞的内皮细胞对照组。使用人血管新生相关蛋白抗体阵列试剂盒检测各组细胞培养上清液中的蛋白因子水平。结果 培养的HUVECs表达VE-钙黏素。与PC3细胞共培养的HUVECs增殖能力比内皮细胞对照组显著增强(P<0.01);以20与50 μmol·L-1木犀草素干预可显著抑制共培养体系中HUVECs的增殖能力(均为P<0.01)。与PC3细胞共培养的HUVECs迁移能力比内皮细胞对照组显著增强(P<0.01);以20与50 μmol·L-1木犀草素干预可显著抑制共培养体系中HUVECs的迁移能力(均为P<0.01)。共培养体系细胞培养上清液中多种血管新生相关蛋白分泌水平显著高于内皮细胞对照组。以20 μmol·L-1木犀草素干预可显著抑制共培养体系细胞培养上清液中白介素-8(IL-8)、血管内皮生长因子(VEGF)及单核细胞趋化蛋白-1(MCP-1)的分泌水平(均为P<0.01)。结论 木犀草素可能通过抑制肿瘤-内皮细胞共培养体系中IL-8、VEGF及MCP-1的分泌抑制内皮细胞的增殖与迁移。

Abstract

OBJECTIVE To explore the effects of luteolin on the proliferation and migration of the endothelial cells co-cultured with cancer cells and its molecular mechanism. METHODS Human umbilical vein endothelial cells (HUVECs) were primarily isolated and identified by the expression of VE-cadherin. Cancer-endothelial cell co-culture model was established using the Transwell system. The effects of luteolin at different concentrations (0 [Co-culture control], 20 and 50 μmol·L-1) on the proliferation and migration of HUVECs in the co-culture system were determined. A HUVECs control group removed of prostate cancer PC3 cells was also included. Human angiogenesis antibody array kit was used to assay the secretion levels of various protein factors in each group. RESULTS VE-cadherin was expressed on all the cultured HUVECs. Increased proliferation ability was found in the HUVECs co-cultured with PC3 cells compared with that in HUVECs control group (P<0.01). Treatment with 20 or 50 μmol·L-1 luteolin significantly inhibited the proliferation ability of the HUVECs in co-culture system (both P<0.01). Increased migration ability was found in the HUVECs co-cultured with PC3 cells compared with that in HUVECs control group (P<0.01). Treatment with 20 or 50 μmol·L-1 luteolin significantly inhibited the migration ability of the HUVECs in co-culture system (both P<0.01). Secretion levels of multiple angiogenesis-related proteins in the cultural supernatant of co-culture system were significantly increased compared with those in HUVECs control group. Treatment with 20 μmol·L-1 luteolin significantly inhibited the secretion levels of interleukin-8 (IL-8), vascular endothelial growth factor (VEGF) and monocyte chemoattractant protein-1(MCP-1) in the cultural supernatant of co-culture system (all P<0.01). CONCLUSION Luteolin may inhibit the proliferation and migration of endothelial cells via suppressing the secretions of IL-8, VEGF and MCP-1 in cancer-endothelial co-culture system.

关键词

木犀草素 / 内皮细胞 / 前列腺癌 / 血管新生 / 迁移

Key words

luteolin / endothelial cell / prostate cancer / angiogenesis / migration

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平耀东, 梁泰刚, 张艳华. 木犀草素抑制与前列腺癌PC3细胞共培养的内皮细胞增殖、迁移及其分子机制[J]. 中国药学杂志, 2018, 53(7): 508-512 https://doi.org/10.11669/cpj.2018.07.006
PING Yao-dong, LIANG Tai-gang, ZHANG Yan-hua. Inhibition Effect of Luteolin on the Proliferation and Migration of the Endothelial Cells Co-Cultured with Cancer Cells and Its Molecular Mechanism[J]. Chinese Pharmaceutical Journal, 2018, 53(7): 508-512 https://doi.org/10.11669/cpj.2018.07.006
中图分类号： R965   

参考文献

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基金

国家自然科学基金项目资助(81101687);山西省中药资源产业化及产业国际化协同创新中心项目资助(HQXTCXZX2016-021)
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